Forkhead Box K2 antibody (AA 491-540)

Catalog No. ABIN6736720

This Rabbit Polyclonal antibody specifically detects Forkhead Box K2 in WB, IHC and IHC (p). It exhibits reactivity toward Human and Rabbit.

Quick Overview for Forkhead Box K2 antibody (AA 491-540) (ABIN6736720)

Target: Forkhead Box K2 (FOXK2)

Reactivity: Human, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Forkhead Box K2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Forkhead Box K2 Antibody

Binding Specificity: AA 491-540

Brand: IHC-plus™

Specificity: Human FOXK2

Predicted Reactivity: Percent identity by BLAST analysis: Human (100%) Bovine, Horse, Pig (92%) Mouse (90%) Rat, Chicken, Xenopus (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa491-540 of human FOXK2 (Q01167, NP_004505). Percent identity by BLAST analysis: Human, Chimpanzee, Opossum (100%), Gibbon, Monkey, Galago, Marmoset, Bovine, Bat, Horse, Pig (92%), Mouse, Rat, Elephant (90%), Turkey, Chicken, Xenopus (85%), Gorilla (81%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: After adding water, will consist of PBS buffer with 2 % sucrose

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for Forkhead Box K2

Target: Forkhead Box K2 (FOXK2)

Alternative Name: FOXK2 / ILF

Background: Name/Gene ID: FOXK2

Synonyms: FOXK2, ILF1, ILF-1, Forkhead box K2, ILF, Forkhead box protein K2

Gene ID: 3607

NCBI Accession: NP_004505

UniProt: Q01167