KIF23 antibody (AA 32-81)

Catalog No. ABIN6736733

The Rabbit Polyclonal anti-KIF23 antibody has been validated for WB, IHC and IHC (p). It is suitable to detect KIF23 in samples from Human, Mouse, Rat, Horse, Rabbit, Dog, Pig, Hamster and Monkey.

Quick Overview for KIF23 antibody (AA 32-81) (ABIN6736733)

Target: KIF23 (Kinesin Family Member 23 (KIF23))

Reactivity: Human, Mouse, Rat, Horse, Rabbit, Dog, Pig, Hamster, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KIF23 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-KIF23 Antibody

Binding Specificity: AA 32-81

Brand: IHC-plus™

Specificity: Human KIF23 / MKLP1

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Rabbit, Horse, Pig (100%) Bovine (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa32-81 of human KIF23 (Q02241, NP_004847). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Hamster, Elephant, Dog, Rabbit, Horse, Pig (100%), Bovine, Opossum (92%), Platypus (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for KIF23

Target: KIF23 (Kinesin Family Member 23 (KIF23))

Alternative Name: KIF23 / MKLP1

Background: Name/Gene ID: KIF23

Synonyms: KIF23, Kinesin family member 23, Kinesin-like protein KIF23, Mitotic kinesin-like 1, MKLP1, MKLP-1, Kinesin-like protein 5, KNSL5, CHO1, Mitotic kinesin-like protein 1

Gene ID: 9493

NCBI Accession: NP_004847

UniProt: Q02241