NFIA antibody (AA 348-397)

Catalog No. ABIN6736739

The Rabbit Polyclonal anti-NFIA antibody has been validated for WB, IHC and IHC (p). It is suitable to detect NFIA in samples from Human, Mouse, Rat, Cow, Horse, Rabbit, Guinea Pig, Pig, Chicken, Monkey and Xenopus laevis.

Quick Overview for NFIA antibody (AA 348-397) (ABIN6736739)

Target: NFIA (Nuclear Factor I/A (NFIA))

Reactivity: Human, Mouse, Rat, Cow, Horse, Rabbit, Guinea Pig, Pig, Chicken, Monkey, Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NFIA antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-NFIA Antibody

Binding Specificity: AA 348-397

Brand: IHC-plus™

Specificity: Human NFIA

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Bovine, Rabbit, Horse, Guinea pig, Chicken (100%) Zebrafish (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa348-397 of human NFIA (Q8TA97, NP_005586). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Bovine, Rabbit, Horse, Pig, Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Platypus, Xenopus (100%), Zebrafish (92%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: After adding water, will consist of PBS buffer with 2 % sucrose

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for NFIA

Target: NFIA (Nuclear Factor I/A (NFIA))

Alternative Name: NFIA / Nuclear Factor 1

Background: Name/Gene ID: NFIA

Synonyms: NFIA, CTF, NF1-A, NF-I/A, NFI-L, Nuclear Factor 1, TGGCA-binding protein, NFI-A, Nuclear factor 1 A-type, KIAA1439, Nuclear factor 1/A, Nuclear factor I/A

Gene ID: 4774

NCBI Accession: NP_005586

UniProt: Q12857