Nucleobindin 1 antibody (C-Term)

Catalog No. ABIN6736741

The Rabbit Polyclonal anti-Nucleobindin 1 antibody has been validated for WB, IHC and IHC (p). It is suitable to detect Nucleobindin 1 in samples from Human.

Quick Overview for Nucleobindin 1 antibody (C-Term) (ABIN6736741)

Target: Nucleobindin 1 (NUCB1)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Nucleobindin 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Nucleobindin 1 Antibody

Binding Specificity: C-Term

Brand: IHC-plus™

Specificity: Human NUCB1

Predicted Reactivity: Percent identity by BLAST analysis: Human (100%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from C-Terminus of human NUCB1 (Q02818, NP_006175). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan (100%), Monkey (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: After adding water, will consist of PBS buffer with 2 % sucrose

Concentration: Lot specific

Buffer: Lyophilized.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for Nucleobindin 1

Target: Nucleobindin 1 (NUCB1)

Alternative Name: NUCB1 / Nucleobindin

Background: Name/Gene ID: NUCB1

Synonyms: NUCB1, CALNUC, Nucleobindin-1, Nucleobindin 1, NUC, Nucleobindin

Gene ID: 4924

NCBI Accession: NP_006175

UniProt: Q02818