Recoverin antibody (AA 131-180)

Catalog No. ABIN6736750

This anti-Recoverin antibody is a Rabbit Polyclonal antibody detecting Recoverin in WB, IHC and IHC (p). Suitable for Human, Rat, Cow and Dog.

Quick Overview for Recoverin antibody (AA 131-180) (ABIN6736750)

Target: Recoverin (RCVRN)

Reactivity: Human, Rat, Cow, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Recoverin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Recoverin Antibody

Binding Specificity: AA 131-180

Brand: IHC-plus™

Specificity: Human RCVRN / Recoverin

Predicted Reactivity: Percent identity by BLAST analysis: Human (100%) Bovine, Frog (85%) Rat (84%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa131-180 of human RCVRN (Q53XL0, NP_002894). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Marmoset (100%), Monkey (92%), Bovine, Horse, Pig, Frog (85%), Rat (84%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: After adding water, will consist of PBS buffer with 2 % sucrose

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for Recoverin

Target: Recoverin (RCVRN)

Alternative Name: Recoverin

Background: Name/Gene ID: RCVRN

Synonyms: RCVRN, Recoverin, Protein CAR, RCV1

Gene ID: 5957

NCBI Accession: NP_002894

UniProt: P35243

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling, Phototransduction