SOX11 antibody (N-Term)

Catalog No. ABIN214147

This Rabbit Polyclonal antibody specifically detects SOX11 in WB, IHC and IHC (p). It exhibits reactivity toward Human, Rat, Mouse, Guinea Pig, Horse, Pig and Monkey.

Quick Overview for SOX11 antibody (N-Term) (ABIN214147)

Target: SOX11 (SRY (Sex Determining Region Y)-Box 11 (SOX11))

Reactivity: Human, Rat, Mouse, Guinea Pig, Horse, Pig, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SOX11 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-SOX11 Antibody

Binding Specificity: N-Term

Brand: IHC-plus™

Specificity: Human SOX11

Predicted Reactivity: Percent identity by BLAST analysis: Human, Horse, Pig, Guinea pig (100%) Rabbit (92%) Mouse, Rat (85%).

Purification: Affinity purified

Immunogen: Synthetic peptide from N-Terminus of human SOX11 (P35716, NP_003099). Percent identity by BLAST analysis: Human, Gorilla, Monkey, Galago, Marmoset, Horse, Pig, Guinea pig (100%), Rabbit (92%), Mouse, Rat, Elephant (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute with 50 μL sterile ddH2O.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for SOX11

Target: SOX11 (SRY (Sex Determining Region Y)-Box 11 (SOX11))

Alternative Name: SOX11

Background: Name/Gene ID: SOX11
Family: Transcription factor

Synonyms: SOX11, SRY-related HMG-box gene 11, Transcription factor SOX-11

Gene ID: 6664

NCBI Accession: NP_003099

UniProt: P35716

Pathways: Tube Formation