SOX3 antibody (AA 80-129)

Catalog No. ABIN6736758

The Rabbit Polyclonal anti-SOX3 antibody (ABIN6736758) specifically detects SOX3 in WB, IHC and IHC (p). The antibody is reactive with Human, Mouse, Dog and Rabbit samples.

Quick Overview for SOX3 antibody (AA 80-129) (ABIN6736758)

Target: SOX3 (SRY (Sex Determining Region Y)-Box 3 (SOX3))

Reactivity: Human, Mouse, Dog, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SOX3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-SOX3 Antibody

Binding Specificity: AA 80-129

Specificity: Human SOX3

Predicted Reactivity: Percent identity by BLAST analysis: Human (100%) Mouse, Dog, Rabbit (84%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa80-129 of human SOX3 (P41225, NP_005625). Percent identity by BLAST analysis: Human, Gorilla, Gibbon (100%), Monkey, Galago, Marmoset (92%), Mouse, Rat, Dog, Rabbit (84%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: After adding water, will consist of PBS buffer with 2 % sucrose

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for SOX3

Target: SOX3 (SRY (Sex Determining Region Y)-Box 3 (SOX3))

Alternative Name: SOX3

Background: Name/Gene ID: SOX3
Family: Transcription factor

Synonyms: SOX3, GHDX, MRGH, SOXB, Panhypopituitarism, PHPX, Sry-box3, PHP, Transcription factor SOX-3

Gene ID: 6658

NCBI Accession: NP_005625

UniProt: P41225