MED16 antibody (AA 171-220)

Catalog No. ABIN6737649

The Rabbit Polyclonal anti-MED16 antibody has been validated for WB. It is suitable to detect MED16 in samples from Human, Mouse, Rat, Dog, Cow, Guinea Pig, Horse, Monkey, Pig and Bat.

Quick Overview for MED16 antibody (AA 171-220) (ABIN6737649)

Target: MED16 (Mediator Complex Subunit 16 (MED16))

Reactivity: Human, Mouse, Rat, Dog, Cow, Guinea Pig, Horse, Monkey, Pig, Bat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MED16 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-MED16 Antibody

Binding Specificity: AA 171-220

Specificity: Human MED16 / THRAP5

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Pig, Guinea pig (100%) Chicken (92%) Xenopus (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa171-220 of human MED16 (Q9Y2X0, NP_005472). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Dog, Bovine, Bat, Horse, Pig, Guinea pig (100%), Opossum, Turkey, Chicken (92%), Zebra finch, Xenopus (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB

Usage: ELISA titer using peptide based assay: 1:312500. Western Blot: Suggested dilution at 0.5 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for MED16

Target: MED16 (Mediator Complex Subunit 16 (MED16))

Alternative Name: MED16 / THRAP5

Background: Name/Gene ID: MED16

Synonyms: MED16, DRIP92, Mediator complex subunit 16, TRAP95, THRAP5

Gene ID: 10025

NCBI Accession: NP_005472

UniProt: Q9Y2X0

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Nuclear Hormone Receptor Binding, Regulation of Lipid Metabolism by PPARalpha