ISL1 antibody (AA 102-151)

Catalog No. ABIN6737674

This anti-ISL1 antibody is a Rabbit Polyclonal antibody detecting ISL1 in WB. Suitable for Human, Mouse, Rat, Zebrafish (Danio rerio), Cow, Dog, Guinea Pig, Rabbit, Sheep, Chicken, Hamster, Monkey, Pig and Xenopus laevis.

Quick Overview for ISL1 antibody (AA 102-151) (ABIN6737674)

Target: ISL1 (ISL LIM Homeobox 1 (ISL1))

Reactivity: Human, Mouse, Rat, Zebrafish (Danio rerio), Cow, Dog, Guinea Pig, Rabbit, Sheep, Chicken, Hamster, Monkey, Pig, Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ISL1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-ISL1 Antibody

Binding Specificity: AA 102-151

Specificity: Human ISL1 / Islet 1

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Rabbit, Pig, Chicken, Xenopus (100%) Horse (92%) Zebrafish (84%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa102-151 of human ISL1 (P61371, NP_002193). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Galago, Marmoset, Mouse, Rat, Sheep, Hamster, Elephant, Dog, Bovine, Rabbit, Pig, Opossum, Guinea pig, Zebra finch, Chicken, Platypus, Lizard, Xenopus, Stickleback (100%), Horse (92%), Salmon, Zebrafish (84%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB

Usage: ELISA titer using peptide based assay: 1:312500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Not recommended for: IHC-P

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for ISL1

Target: ISL1 (ISL LIM Homeobox 1 (ISL1))

Alternative Name: ISLET-1 / ISL1

Background: Name/Gene ID: ISL1

Synonyms: ISL1, Isl-1, ISLET1, ISL LIM homeobox 1, Islet-1

Gene ID: 3670

NCBI Accession: NP_002193

UniProt: P61371

Pathways: Positive Regulation of Peptide Hormone Secretion, Intracellular Steroid Hormone Receptor Signaling Pathway, Peptide Hormone Metabolism, Regulation of Intracellular Steroid Hormone Receptor Signaling, Nuclear Hormone Receptor Binding, Chromatin Binding