RAD18 antibody (AA 197-246)

Catalog No. ABIN6738067

This Rabbit Polyclonal antibody specifically detects RAD18 in WB. It exhibits reactivity toward Human, Rat, Mouse, Pig, Rabbit, Cow, Dog, Horse and Bat.

Quick Overview for RAD18 antibody (AA 197-246) (ABIN6738067)

Target: RAD18 (E3 ubiquitin-protein ligase RAD18 (RAD18))

Reactivity: Human, Rat, Mouse, Pig, Rabbit, Cow, Dog, Horse, Bat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RAD18 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-RAD18 Antibody

Binding Specificity: AA 197-246

Specificity: Human RAD18

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Bovine, Rabbit, Horse, Pig (100%) Guinea pig (92%) Dog, Chicken (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa197-246 of human RAD18 (Q9NS91, NP_064550). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Mouse, Rat, Elephant, Bovine, Bat, Rabbit, Horse, Pig (100%), Marmoset, Hamster, Panda, Opossum, Guinea pig (92%), Galago, Dog, Turkey, Zebra finch, Chicken, Sablefish, Stickleback (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB

Usage: ELISA titer using peptide based assay: 1:312500. Western Blot: Suggested dilution at 0.5 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for RAD18

Target: RAD18 (E3 ubiquitin-protein ligase RAD18 (RAD18))

Alternative Name: RAD18

Background: Name/Gene ID: RAD18

Synonyms: RAD18, HHR18, HRAD18, RNF73, RAD18 homolog (S. cerevisiae), RAD18, S. cerevisiae, homolog, RING finger protein 73

Gene ID: 56852

NCBI Accession: NP_064550