NrCAM antibody (N-Term)

Catalog No. ABIN6738134

The Rabbit Polyclonal anti-NrCAM antibody (ABIN6738134) specifically detects NrCAM in WB. The antibody is reactive with Human, Mouse, Rat, Dog, Cow, Guinea Pig, Horse, Zebrafish (Danio rerio), Monkey and Pig samples.

Quick Overview for NrCAM antibody (N-Term) (ABIN6738134)

Target: NrCAM (NRCAM) (Neuronal Cell Adhesion Molecule (NRCAM))

Reactivity: Human, Mouse, Rat, Dog, Cow, Guinea Pig, Horse, Zebrafish (Danio rerio), Monkey, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NrCAM antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-NrCAM Antibody

Binding Specificity: N-Term

Specificity: Human NRCAM

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Horse, Pig, Guinea pig, Zebrafish (100%) Rabbit, Chicken (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from N-Terminus of human NRCAM (Q4KMQ7). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bovine, Horse, Pig, Opossum, Guinea pig, Lizard, Zebrafish (100%), Bat, Rabbit, Turkey, Zebra finch, Chicken, Stickleback (92%), Platypus (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB

Usage: ELISA titer using peptide based assay: 1:62500. Western Blot: Suggested dilution at 0.5 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Not recommended for: IHC-P

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for NrCAM

Target: NrCAM (NRCAM) (Neuronal Cell Adhesion Molecule (NRCAM))

Alternative Name: NRCAM

Background: Name/Gene ID: NRCAM
Family: Immunoglobulin

Synonyms: NRCAM, Bravo, HBravo, Neuronal surface protein Bravo, KIAA0343, Ng-CAM-related, Nr-CAM

Gene ID: 4897

UniProt: Q92823

Pathways: Regulation of Cell Size