MAP4K1 antibody (AA 218-267)

Catalog No. ABIN6738201

The Rabbit Polyclonal anti-MAP4K1 antibody has been validated for WB. It is suitable to detect MAP4K1 in samples from Human, Mouse, Rat, Dog, Pig, Horse and Bat.

Quick Overview for MAP4K1 antibody (AA 218-267) (ABIN6738201)

Target: MAP4K1 (Mitogen-Activated Protein Kinase Kinase Kinase Kinase 1 (MAP4K1))

Reactivity: Human, Mouse, Rat, Dog, Pig, Horse, Bat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MAP4K1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-MAP4K1 Antibody

Binding Specificity: AA 218-267

Specificity: Human MAP4K1

Predicted Reactivity: Percent identity by BLAST analysis: Mouse, Rat, Dog, Horse, Pig (100%) Bovine (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa218-267 of human MAP4K1 (NP_001036065). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Galago, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bat, Horse, Pig, Opossum (100%), Monkey, Bovine, Guinea pig (92%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB

Usage: ELISA titer using peptide based assay: 1:312500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for MAP4K1

Target: MAP4K1 (Mitogen-Activated Protein Kinase Kinase Kinase Kinase 1 (MAP4K1))

Alternative Name: MAP4K1 / HPK1

Background: Name/Gene ID: MAP4K1
Subfamily: KHS
Family: Protein Kinase

Synonyms: MAP4K1, HPK1, MEKKK 1, MEK kinase kinase 1, MHPK1

Gene ID: 11184

NCBI Accession: NP_001036065

UniProt: Q92918

Pathways: TCR Signaling, Signaling of Hepatocyte Growth Factor Receptor