Nanog antibody (AA 21-70)

Catalog No. ABIN6738410

The Rabbit Polyclonal anti-Nanog antibody has been validated for WB. It is suitable to detect Nanog in samples from Human, Pig, Cow and Sheep.

Quick Overview for Nanog antibody (AA 21-70) (ABIN6738410)

Target: Nanog (NANOG) (Nanog Homeobox (NANOG))

Reactivity: Human, Pig, Cow, Sheep

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Nanog antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Nanog Antibody

Binding Specificity: AA 21-70

Specificity: Human Nanog

Predicted Reactivity: Percent identity by BLAST analysis: Human, Sheep, Bovine, Pig (100%) Dog (91%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa21-70 of human NANOG (Q9H9S0, NP_079141). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Sheep, Bovine, Pig (100%), Marmoset, Dog, Horse (91%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB

Usage: ELISA titer using peptide based assay: 1:312500. Western Blot: Suggested dilution at 0.25 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for Nanog

Target: Nanog (NANOG) (Nanog Homeobox (NANOG))

Alternative Name: NANOG

Background: Name/Gene ID: NANOG

Synonyms: NANOG, Nanog homeobox, NANOGP8, HNanog, Homeobox protein NANOG

Gene ID: 79923

NCBI Accession: NP_079141

UniProt: Q9H9S0

Pathways: Stem Cell Maintenance