PRAS40 antibody (AA 202-251)

Catalog No. ABIN6738417

The Rabbit Polyclonal anti-PRAS40 antibody has been validated for WB. It is suitable to detect PRAS40 in samples from Human, Mouse, Rat, Cow, Guinea Pig, Horse, Pig, Bat, Hamster, Monkey.

Quick Overview for PRAS40 antibody (AA 202-251) (ABIN6738417)

Target: PRAS40 (AKT1S1) (AKT1 Substrate 1 (Proline-Rich) (AKT1S1))

Reactivity: Human, Mouse, Rat, Cow, Guinea Pig, Horse, Pig, Bat, Hamster, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PRAS40 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-PRAS40 Antibody

Binding Specificity: AA 202-251

Specificity: Human AKT1S1 / PRAS40

Predicted Reactivity: Percent identity by BLAST analysis: Mouse, Rat, Bovine, Pig, Guinea pig (100%) Dog (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa202-251 of human AKT1S1 (Q96B36, NP_115751). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Hamster, Panda, Bovine, Bat, Horse, Pig, Guinea pig (100%), Dog (92%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB

Usage: ELISA titer using peptide based assay: 1:62500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for PRAS40

Target: PRAS40 (AKT1S1) (AKT1 Substrate 1 (Proline-Rich) (AKT1S1))

Alternative Name: AKT1S1 / PRAS40

Background: Name/Gene ID: AKT1S1

Synonyms: AKT1S1, PRAS40, Proline-rich AKT1 substrate 1, Lobe

Gene ID: 84335

NCBI Accession: NP_115751

UniProt: Q96B36

Pathways: Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Cell Size, Autophagy, BCR Signaling, Warburg Effect