PRAS40 antibody (AA 152-276)

Catalog No. ABIN6136741

The Rabbit Polyclonal anti-PRAS40 antibody (ABIN6136741) specifically detects PRAS40 in WB, IHC and IF. The antibody is reactive with Human samples.

Quick Overview for PRAS40 antibody (AA 152-276) (ABIN6136741)

Target: PRAS40 (AKT1S1) (AKT1 Substrate 1 (Proline-Rich) (AKT1S1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PRAS40 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-PRAS40 Antibody

Binding Specificity: AA 152-276

Sequence: DEDATLQDLP PFCESDPEST DDGSLSEETP AGPPTCSVPP ASALPTQQYA KSLPVSVPVW GFKEKRTEAR SSDEENGPPS SPDLDRIAAS MRALVLREAE DTQVFGDLPR PRLNTSDFQK LKRKY

Cross-Reactivity: Human, Mouse, Rat

Characteristics: Polyclonal Antibodies

Purification: Affinity purification

Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 152-276 of human AKT1S1/PRAS40 (NP_115751.3).

Isotype: IgG

Application Details

Application Notes: WB,1:500 - 1:2000,IHC,1:50 - 1:100,IF,1:50 - 1:100

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide,50 % glycerol, pH 7.3.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze / thaw cycles.

Target Details for PRAS40

Target: PRAS40 (AKT1S1) (AKT1 Substrate 1 (Proline-Rich) (AKT1S1))

Alternative Name: AKT1S1

Background: AKT1S1 is a proline-rich substrate of AKT (MIM 164730) that binds 14-3-3 protein (see YWHAH, MIM 113508) when phosphorylated (Kovacina et al., 2003 [PubMed 12524439]).[supplied by OMIM, Mar 2008],AKT1S1,Lobe,PRAS40,Epigenetics & Nuclear Signaling,Translation Control,Regulation of eIF4 and p70 S6 Kinase,Signal Transduction,Kinase,PI3K-Akt Signaling Pathway,mTOR Signaling Pathway,Cell Biology & Developmental Biology,Apoptosis,Autophagy,Cell Cycle,Endocrine & Metabolism,Insulin Receptor Signaling Pathway,Immunology & Inflammation,T Cell Receptor Signaling Pathway,Neuroscience,Akt downstream targets,AKT1S1

Molecular Weight: 13 kDa/27 kDa/29 kDa

Gene ID: 84335

UniProt: Q96B36

Pathways: Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Cell Size, Autophagy, BCR Signaling, Warburg Effect