ENO3 antibody (AA 21-70)

Catalog No. ABIN6738428

This Rabbit Polyclonal antibody specifically detects ENO3 in WB. It exhibits reactivity toward Human.

Quick Overview for ENO3 antibody (AA 21-70) (ABIN6738428)

Target: ENO3 (Enolase 3 (Beta, Muscle) (ENO3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ENO3 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-ENO3 Antibody

Binding Specificity: AA 21-70

Specificity: Human ENO3

Predicted Reactivity: Percent identity by BLAST analysis: Human (100%) Mouse, Dog, Pig, Xenopus (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa21-70 of human ENO3 (P13929, NP_001967). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey (100%), Orangutan, Galago, Marmoset, Mouse, Rat, Goat, Hamster, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Xenopus, Salmon, Paramecium (92%), Sablefish, Slime mold (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB

Usage: ELISA titer using peptide based assay: 1:12500. Western Blot: Suggested dilution at 0.25 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for ENO3

Target: ENO3 (Enolase 3 (Beta, Muscle) (ENO3))

Alternative Name: ENO3 / Enolase 3

Background: Name/Gene ID: ENO3

Synonyms: ENO3, Beta-enolase, Enolase 3, Enolase 3 (beta, muscle), Enolase 3, (beta, muscle), Muscle-specific enolase, MSE, Skeletal muscle enolase, GSD13

Gene ID: 2027

NCBI Accession: NP_001967

UniProt: P13929