HPRT1 antibody (AA 110-159)

Catalog No. ABIN6738466

The Rabbit Polyclonal anti-HPRT1 antibody has been validated for WB. It is suitable to detect HPRT1 in samples from Human, Mouse, Rat, Zebrafish (Danio rerio), Dog, Cow, Guinea Pig, Horse, Rabbit and Pig.

Quick Overview for HPRT1 antibody (AA 110-159) (ABIN6738466)

Target: HPRT1 (Hypoxanthine phosphoribosyltransferase 1 (HPRT1))

Reactivity: Human, Mouse, Rat, Zebrafish (Danio rerio), Dog, Cow, Guinea Pig, Horse, Rabbit, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HPRT1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-HPRT1 Antibody

Binding Specificity: AA 110-159

Specificity: Human HPRT1 / HPRT

Predicted Reactivity: Percent identity by BLAST analysis: Human, Rat, Bovine, Rabbit, Horse, Pig, Guinea pig, Chicken, Xenopus, Zebrafish (100%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa110-159 of human HPRT1 (P00492, NP_000185). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Hamster, Elephant, Dog, Cat, Bovine, Bat, Rabbit, Horse, Pig, Guinea pig, Turkey, Chicken, Platypus, Xenopus, Zebrafish (100%), Catfish, Salmon, Smelt, Stickleback (92%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB

Usage: ELISA titer using peptide based assay: 1:12500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for HPRT1

Target: HPRT1 (Hypoxanthine phosphoribosyltransferase 1 (HPRT1))

Alternative Name: HPRT1 / HPRT

Background: Name/Gene ID: HPRT1

Synonyms: HPRT1, HGPRT, HGPRTase, HPRT

Gene ID: 3251

NCBI Accession: NP_000185

UniProt: P00492

Pathways: Ribonucleoside Biosynthetic Process