MAP4K2 antibody (AA 216-265)

Catalog No. ABIN6738482

This Rabbit Polyclonal antibody specifically detects MAP4K2 in WB. It exhibits reactivity toward Human, Mouse, Rat, Cow, Guinea Pig, Zebrafish (Danio rerio), Horse and Bat.

Quick Overview for MAP4K2 antibody (AA 216-265) (ABIN6738482)

Target: MAP4K2 (Mitogen-Activated Protein Kinase Kinase Kinase Kinase 2 (MAP4K2))

Reactivity: Human, Mouse, Rat, Cow, Guinea Pig, Zebrafish (Danio rerio), Horse, Bat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MAP4K2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-MAP4K2 Antibody

Binding Specificity: AA 216-265

Specificity: Human MAP4K2 / GCK

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Bovine, Horse (100%) Dog, Pig (92%) Guinea pig (84%) Zebrafish (83%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa216-265 of human MAP4K2 (Q12851, NP_004570). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Baboon, Galago, Mouse, Rat, Elephant, Bovine, Bat, Horse (100%), Monkey, Marmoset, Panda, Dog, Pig, Armadillo, Salmon (92%), Rabbit, Guinea pig, Zebrafish (84%), Orangutan, Opossum, Zebra finch, Chicken, Platypus, Lizard, Xenopus, Stickleback (83%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB

Usage: ELISA titer using peptide based assay: 1:312500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for MAP4K2

Target: MAP4K2 (Mitogen-Activated Protein Kinase Kinase Kinase Kinase 2 (MAP4K2))

Alternative Name: GCK / Germinal Center Kinase

Background: Name/Gene ID: MAP4K2
Subfamily: KHS
Family: Protein Kinase

Synonyms: MAP4K2, BL44, GC kinase, Rab8 interacting protein, RAB8IP, Rab8-interacting protein, Germinal center kinase, MEK kinase kinase 2, MEKKK 2

Gene ID: 5871

NCBI Accession: NP_004570

UniProt: Q12851