POLR3B antibody (AA 866-915)

Catalog No. ABIN6738508

The Rabbit Polyclonal anti-POLR3B antibody has been validated for WB. It is suitable to detect POLR3B in samples from Human, Mouse, Rat, Dog, Cow, Rabbit, Zebrafish (Danio rerio), Pig, Horse, Monkey, Guinea Pig, Drosophila melanogaster, Chicken, Hamster, Bat and Xenopus laevis.

Quick Overview for POLR3B antibody (AA 866-915) (ABIN6738508)

Target: POLR3B (Polymerase (RNA) III (DNA Directed) Polypeptide B (POLR3B))

Reactivity: Human, Mouse, Rat, Dog, Cow, Rabbit, Zebrafish (Danio rerio), Pig, Horse, Monkey, Guinea Pig, Drosophila melanogaster, Chicken, Hamster, Bat, Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POLR3B antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-POLR3B Antibody

Binding Specificity: AA 866-915

Specificity: Human POLR3B

Predicted Reactivity: Percent identity by BLAST analysis: Human, Rat, Dog, Bovine, Rabbit, Horse, Pig, Guinea pig, Chicken, Zebrafish (100%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa866-915 of human POLR3B (Q9NW08, NP_060552). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Zebra finch, Chicken, Platypus, Lizard, Xenopus, Salmon, Stickleback, Zebrafish, Drosophila, Beetle (100%), Sea squirt, Slime mold, Rice, Arabidopsis (92%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB

Usage: ELISA titer using peptide based assay: 1:12500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for POLR3B

Target: POLR3B (Polymerase (RNA) III (DNA Directed) Polypeptide B (POLR3B))

Alternative Name: POLR3B

Background: Name/Gene ID: POLR3B

Synonyms: POLR3B, HLD8, RPC2, C128, RNA polymerase III subunit C2

Gene ID: 55703

NCBI Accession: NP_060552