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MANEA antibody (AA 218-267)

The Rabbit Polyclonal anti-MANEA antibody has been validated for WB. It is suitable to detect MANEA in samples from Human, Mouse, Rat, Dog, Horse and Zebrafish (Danio rerio).
Catalog No. ABIN6738631

Quick Overview for MANEA antibody (AA 218-267) (ABIN6738631)

Target

See all MANEA Antibodies
MANEA (Mannosidase, Endo-alpha (MANEA))

Reactivity

  • 5
  • 3
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
Human, Mouse, Rat, Dog, Horse, Zebrafish (Danio rerio)

Host

  • 5
Rabbit

Clonality

  • 5
Polyclonal

Conjugate

  • 5
This MANEA antibody is un-conjugated

Application

  • 5
  • 1
  • 1
Western Blotting (WB)
  • Binding Specificity

    • 2
    • 1
    AA 218-267

    Specificity

    Human MANEA

    Predicted Reactivity

    Percent identity by BLAST analysis: Human, Mouse, Rat, Dog (100%) Xenopus (90%) Horse (85%) Chicken (83%).

    Purification

    Immunoaffinity purified

    Immunogen

    Synthetic peptide located between aa218-267 of human MANEA (Q5SRI9, NP_078917). Percent identity by BLAST analysis: Human, Chimpanzee, Orangutan, Gibbon, Monkey, Galago, Mouse, Rat, Elephant, Panda, Dog (100%), Marmoset, Bat, Pig (92%), Xenopus (91%), Hamster, Horse (85%), Opossum (84%), Turkey, Zebra finch, Chicken (83%).

    Type of Immunogen: Synthetic peptide

    Isotype

    IgG
  • Application Notes

    Approved: WB

    Usage: ELISA titer using peptide based assay: 1:312500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

    Comment

    Target Species of Antibody: Human

    Restrictions

    For Research Use only
  • Format

    Lyophilized

    Reconstitution

    Distilled Water.

    Concentration

    Lot specific

    Buffer

    Lyophilized from PBS with 2 % sucrose

    Handling Advice

    Avoid repeat freeze-thaw cycles.

    Storage

    4 °C,-20 °C

    Storage Comment

    Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
    Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
  • Target

    MANEA (Mannosidase, Endo-alpha (MANEA))

    Alternative Name

    MANEA / ENDO

    Background

    Name/Gene ID: MANEA

    Synonyms: MANEA, ENDO, Endo-alpha mannosidase, Endomannosidase, HEndo, Alpha 1,2-endomannosidase, Mandaselin, Mannosidase, endo-alpha

    Gene ID

    79694

    NCBI Accession

    NP_078917

    UniProt

    Q5SRI9
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