SPRY1 antibody (AA 35-84)

Catalog No. ABIN6738713

This anti-SPRY1 antibody is a Rabbit Polyclonal antibody detecting SPRY1 in WB. Suitable for Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Zebrafish (Danio rerio), Bat, Hamster, Monkey and Pig.

Quick Overview for SPRY1 antibody (AA 35-84) (ABIN6738713)

Target: SPRY1 (Sprouty Homolog 1, Antagonist of FGF Signaling (SPRY1))

Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Zebrafish (Danio rerio), Bat, Hamster, Monkey, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SPRY1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-SPRY1 Antibody

Binding Specificity: AA 35-84

Specificity: Human SPRY1

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine (100%) Chicken (92%) Zebrafish (84%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa35-84 of human SPRY1 (O43609, NP_005832). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Guinea pig, Zebra finch, Zebrafish (100%), Opossum, Turkey, Chicken, Salmon (92%), Xenopus (91%), Lizard (85%), Orangutan, Platypus (83%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB

Usage: ELISA titer using peptide based assay: 1:1562500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for SPRY1

Target: SPRY1 (Sprouty Homolog 1, Antagonist of FGF Signaling (SPRY1))

Alternative Name: SPRY1 / Sprouty 1

Background: Name/Gene ID: SPRY1

Synonyms: SPRY1, HSPRY1, Protein sprouty homolog 1, Spry-1

Gene ID: 10252

NCBI Accession: NP_005832

UniProt: O43609

Pathways: EGFR Signaling Pathway, EGFR Downregulation