EIF5 antibody (N-Term)

Catalog No. ABIN6738843

The Rabbit Polyclonal anti-EIF5 antibody has been validated for WB. It is suitable to detect EIF5 in samples from Human, Mouse, Rat, Cow, Horse, Dog, Guinea Pig, Rabbit, Monkey, Zebrafish (Danio rerio), Pig, Chicken and Xenopus laevis.

Quick Overview for EIF5 antibody (N-Term) (ABIN6738843)

Target: EIF5 (Eukaryotic Translation Initiation Factor 5 (EIF5))

Reactivity: Human, Mouse, Rat, Cow, Horse, Dog, Guinea Pig, Rabbit, Monkey, Zebrafish (Danio rerio), Pig, Chicken, Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EIF5 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-EIF5 Antibody

Binding Specificity: N-Term

Specificity: Human EIF5

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Horse, Chicken, Xenopus, Zebrafish (100%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from N-Terminus of human EIF5 (P55010, NP_892116). Percent identity by BLAST analysis: Human, Chimpanzee, Orangutan, Gibbon, Galago, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bovine, Rabbit, Horse, Pig, Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Platypus, Xenopus, Stickleback, Zebrafish (100%), Trout, Salmon, Beetle (92%), Gorilla, Drosophila (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB

Usage: ELISA titer using peptide based assay: 1:312500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for EIF5

Target: EIF5 (Eukaryotic Translation Initiation Factor 5 (EIF5))

Alternative Name: EIF5 / EIF-5A

Background: Name/Gene ID: EIF5

Synonyms: EIF5, EIF-5A, EIF-5

Gene ID: 1983

NCBI Accession: NP_892116

UniProt: P55010