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HMBS antibody (AA 146-195)

This anti-HMBS antibody is a Rabbit Polyclonal antibody detecting HMBS in WB. Suitable for Human.
Catalog No. ABIN6738946

Quick Overview for HMBS antibody (AA 146-195) (ABIN6738946)

Target

See all HMBS Antibodies
HMBS (Hydroxymethylbilane Synthase (HMBS))

Reactivity

  • 66
  • 35
  • 35
  • 3
  • 3
  • 3
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
Human

Host

  • 59
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  • 1
Rabbit

Clonality

  • 51
  • 15
Polyclonal

Conjugate

  • 31
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  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
This HMBS antibody is un-conjugated

Application

  • 44
  • 14
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  • 12
  • 8
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  • 4
  • 4
  • 1
  • 1
  • 1
Western Blotting (WB)
  • Binding Specificity

    • 8
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    • 3
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    • 1
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    • 1
    AA 146-195

    Specificity

    Human HMBS

    Predicted Reactivity

    Percent identity by BLAST analysis: Human, Rabbit (100%) Mouse, Rat, Dog, Goat, Pig (92%) Chicken, Zebrafish (90%) Xenopus (85%).

    Purification

    Immunoaffinity purified

    Immunogen

    Synthetic peptide located between aa146-195 of human HMBS (P08397, NP_000181). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset, Elephant, Rabbit, Opossum, Guinea pig (100%), Galago, Mouse, Rat, Goat, Panda, Dog, Cat, Bovine, Horse, Pig, Platypus (92%), Turkey, Chicken, Salmon, Smelt, Stickleback, Zebrafish, Sea squirt, Beetle (90%), Bat, Xenopus (85%).

    Type of Immunogen: Synthetic peptide

    Isotype

    IgG
  • Application Notes

    Approved: WB

    Usage: ELISA titer using peptide based assay: 1:312500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

    Comment

    Target Species of Antibody: Human

    Restrictions

    For Research Use only
  • Format

    Lyophilized

    Reconstitution

    Distilled Water.

    Concentration

    Lot specific

    Buffer

    Lyophilized from PBS with 2 % sucrose

    Handling Advice

    Avoid repeat freeze-thaw cycles.

    Storage

    4 °C,-20 °C

    Storage Comment

    Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
    Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
  • Target

    HMBS (Hydroxymethylbilane Synthase (HMBS))

    Alternative Name

    HMBS / PBGD

    Background

    Name/Gene ID: HMBS

    Synonyms: HMBS, Hydroxymethylbilane synthase, PBG-D, PBGD, PORC, Porphyria, acute Chester type, Porphobilinogen deaminase, Pre-uroporphyrinogen synthase, UPS, Uroporphyrinogen I synthetase, Uroporphyrinogen I synthase

    Gene ID

    3145

    NCBI Accession

    NP_000181

    UniProt

    P08397
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