LIF antibody (N-Term)

Catalog No. ABIN6738983

This anti-LIF antibody is a Rabbit Polyclonal antibody detecting LIF in WB. Suitable for Human, Mouse, Rat, Dog, Cow, Guinea Pig, Horse and Monkey.

Quick Overview for LIF antibody (N-Term) (ABIN6738983)

Target: LIF (Leukemia Inhibitory Factor (LIF))

Reactivity: Human, Mouse, Rat, Dog, Cow, Guinea Pig, Horse, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LIF antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-LIF Antibody

Binding Specificity: N-Term

Specificity: Human LIF

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Horse, Guinea pig (100%) Pig (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from N-Terminus of human LIF (P15018, NP_002300). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Panda, Dog, Bovine, Horse, Opossum, Guinea pig (100%), Pig (92%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB

Usage: ELISA titer using peptide based assay: 1:62500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute with 50 μL sterile ddH2O.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for LIF

Target: LIF (Leukemia Inhibitory Factor (LIF))

Alternative Name: LIF

Background: Name/Gene ID: LIF

Synonyms: LIF, DIA, Emfilermin, HILDA, Melanoma-derived LPL inhibitor, MLPLI, Leukemia inhibitory factor, CDF, D factor, Human interleukin in DA cells

Gene ID: 3976

NCBI Accession: NP_002300

UniProt: P15018

Pathways: JAK-STAT Signaling, Positive Regulation of Peptide Hormone Secretion, Negative Regulation of Hormone Secretion, Stem Cell Maintenance, Growth Factor Binding