SIRPA antibody (C-Term)

Catalog No. ABIN6739075

The Rabbit Polyclonal anti-SIRPA antibody has been validated for WB. It is suitable to detect SIRPA in samples from Human, Mouse, Monkey and Horse.

Quick Overview for SIRPA antibody (C-Term) (ABIN6739075)

Target: SIRPA (Signal-Regulatory Protein alpha (SIRPA))

Reactivity: Human, Mouse, Monkey, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SIRPA antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-SIRPA Antibody

Binding Specificity: C-Term

Specificity: Human SIRPA

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse (100%) Bovine (92%) Rat, Pig (90%) Horse (84%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from C-Terminus of human SIRPA (B2R6C3, NP_542970). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Monkey, Marmoset, Mouse (100%), Bovine (92%), Rat, Pig (90%), Horse (84%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB

Usage: ELISA titer using peptide based assay: 1:62500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS, 2 % sucrose.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for SIRPA

Target: SIRPA (Signal-Regulatory Protein alpha (SIRPA))

Alternative Name: CD172A / SIRPA

Background: Name/Gene ID: SIRPA

Synonyms: SIRPA, CD172A, CD172a antigen, Inhibitory receptors shps-1, Inhibitory receptor SHPS-1, Macrophage fusion receptor, MyD-1 antigen, p84, SHPS-1, SHPS1, SIRP, Sirp-alpha-1, Sirp-alpha-3, Sirp-alpha1, MYD1, SIRPalpha, SIRPalpha2, BIT, MFR, MYD-1, PTPNS1, SHP substrate 1, Sirp-alpha-2

Gene ID: 140885

NCBI Accession: NP_542970

UniProt: P78324