TRH antibody (C-Term)

Catalog No. ABIN6739088

The Rabbit Polyclonal anti-TRH antibody is suitable to detect TRH in samples from Human, Cow, Guinea Pig, Dog, Bat, Horse, Monkey and Pig. It has been validated for WB.

Quick Overview for TRH antibody (C-Term) (ABIN6739088)

Target: TRH (Thyrotropin-Releasing Hormone (TRH))

Reactivity: Human, Cow, Guinea Pig, Dog, Bat, Horse, Monkey, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TRH antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-TRH Antibody

Binding Specificity: C-Term

Specificity: Human TRH

Predicted Reactivity: Percent identity by BLAST analysis: Human, Bovine (100%) Zebrafish (92%) Guinea pig (84%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from C-Terminus of human TRH (P20396, NP_009048). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Galago, Elephant, Panda, Dog, Bovine, Bat, Horse, Pig, Medaka (100%), Zebrafish (92%), Turkey (91%), Smelt, Stickleback, Pufferfish (90%), Guinea pig (84%), Zebra finch, Salmon (83%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB

Usage: ELISA titer using peptide based assay: 1:2500. Western Blot: Suggested dilution at 0.5 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS, 2 % sucrose.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for TRH

Target: TRH (Thyrotropin-Releasing Hormone (TRH))

Alternative Name: TRH

Background: Name/Gene ID: TRH

Synonyms: TRH, Thyroliberin, Thyrotropin-releasing hormone, Prothyroliberin

Gene ID: 7200

NCBI Accession: NP_009048

UniProt: P20396

Pathways: Positive Regulation of Peptide Hormone Secretion, Feeding Behaviour