ARHGEF25/GEFT antibody (AA 506-555)

Catalog No. ABIN6739417

The Rabbit Polyclonal anti-ARHGEF25/GEFT antibody has been validated for WB. It is suitable to detect ARHGEF25/GEFT in samples from Rat and Human.

Quick Overview for ARHGEF25/GEFT antibody (AA 506-555) (ABIN6739417)

Target: ARHGEF25/GEFT (ARHGEF25) (Rho Guanine Nucleotide Exchange Factor 25 (ARHGEF25))

Reactivity: Rat, Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ARHGEF25/GEFT antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-ARHGEF25/GEFT Antibody

Binding Specificity: AA 506-555

Specificity: Human ARHGEF25 / GEFT

Predicted Reactivity: Percent identity by BLAST analysis: Rat (84%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa506-555 of human ARHGEF25 (Q86VW2, NP_891992). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey (100%), Galago, Marmoset, Bovine, Pig (92%), Mouse, Rat, Dog, Rabbit, Horse, Guinea pig (84%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB

Usage: ELISA titer using peptide based assay: 1:312500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for ARHGEF25/GEFT

Target: ARHGEF25/GEFT (ARHGEF25) (Rho Guanine Nucleotide Exchange Factor 25 (ARHGEF25))

Alternative Name: ARHGEF25 / GEFT

Background: Name/Gene ID: ARHGEF25

Synonyms: ARHGEF25, GEFT, RhoA/RAC/CDC42 exchange factor, p63RhoGEF, RAC/CDC42 exchange factor

Gene ID: 115557

NCBI Accession: NP_891992

UniProt: Q86VW2