Granulocytes antibody (Biotin)

Catalog No. ABIN6740251

The Mouse Monoclonal anti- antibody has been validated for IHC and IHC (fro). It is suitable to detect in samples from Rat.

Quick Overview for Granulocytes antibody (Biotin) (ABIN6740251)

Target: Granulocytes

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: Biotin

Application: Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: RK-4

Product Details

Specificity: The antigen is localized on the cell surface. The epitope has not been further characterized. Antigen distribution: Isolated cells Only a low percentage of cells from peripheral blood stains positive. Positive on peritoneal cells 18h after thioglycollate injection (95 % granulocytes). Negative on peritoneal cells 4 days after thioglycollate injection. Negative on isolated lymphocytes, monocytes, erythrocytes and platelets. Specificity: Rat: pan-granulocytes,. Other species: human: negative, other species not tested. Tissue staining: RK-4 reaction pattern: Tissues tested Results Bone marrow positive on granulocytes positive on granulocyte precursors positive on segmented cells positive on band cells positive on metamyelocytes positive on myelocytes Lung negative on alveolar macrophages, interstitial tissues are occasionally positive. Kidney negative, on perivascular granulocytes occasionally positive. Skin negative, on perivascular granulocytes occasionally positive. Liver negative, on perivascular granulocytes occasionally positive. Blood vessel wall negative

Purification: Affinity purified

Immunogen: Peritoneal cells

Application Details

Application Notes: Approved: IHC, IHC-Fr (1:400)

Usage: The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.5 mL distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS  pH 7.2, 10 mg/mL BSA, 0.01 % thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 4°C.

Target Details

Target: Granulocytes

Alternative Name: Granulocyte