PAX7 antibody (AA 143-192)

Catalog No. ABIN6740298

The Rabbit Polyclonal anti-PAX7 antibody has been validated for WB. It is suitable to detect PAX7 in samples from Human, Mouse, Rat, Guinea Pig, Horse and Monkey.

Quick Overview for PAX7 antibody (AA 143-192) (ABIN6740298)

Target: PAX7 (Paired Box 7 (PAX7))

Reactivity: Human, Mouse, Rat, Guinea Pig, Horse, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PAX7 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-PAX7 Antibody

Binding Specificity: AA 143-192

Specificity: Human PAX7

Predicted Reactivity: Percent identity by BLAST analysis: Human, Horse (100%) Bovine, Rabbit (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa143-192 of human PAX7 (P23759, NP_002575). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Monkey, Marmoset, Horse (100%), Gibbon, Galago, Elephant, Panda, Bovine, Rabbit (92%), Opossum (85%), Drosophila (84%), Mouse, Rat (75%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:12500.

Not recommended for: IHC-P

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for PAX7

Target: PAX7 (Paired Box 7 (PAX7))

Alternative Name: PAX7

Background: Name/Gene ID: PAX7

Synonyms: PAX7, HUP1, Paired box 7, Paired box gene 7, Paired box homeotic gene 7, Paired box protein Pax-7, RMS2, Paired domain gene 7, PAX7 transcriptional factor, PAX7B

Gene ID: 5081

NCBI Accession: NP_002575

UniProt: P23759