USP3 antibody (AA 180-229)

Catalog No. ABIN6740347

The Rabbit Polyclonal anti-USP3 antibody is suitable to detect USP3 in samples from Human, Rat, Dog, Horse, Pig, Bat and Chicken. It has been validated for WB.

Quick Overview for USP3 antibody (AA 180-229) (ABIN6740347)

Target: USP3 (Ubiquitin Specific Peptidase 3 (USP3))

Reactivity: Human, Rat, Dog, Horse, Pig, Bat, Chicken

Host: Rabbit

Clonality: Polyclonal

Conjugate: This USP3 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-USP3 Antibody

Binding Specificity: AA 180-229

Specificity: Human USP3

Predicted Reactivity: Percent identity by BLAST analysis: Human, Dog, Horse, Pig, Chicken (100%) Mouse, Rat, Bovine, Xenopus (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa180-229 of human USP3 (Q9Y6I4, NP_006528). Percent identity by BLAST analysis: Human, Chimpanzee, Gibbon, Monkey, Galago, Rat, Elephant, Panda, Dog, Bat, Horse, Pig, Opossum, Turkey, Zebra finch, Chicken, Platypus (100%), Marmoset, Mouse, Bovine, Xenopus (92%), Rabbit, Guinea pig (85%), Zebrafish (84%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:12500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for USP3

Target: USP3 (Ubiquitin Specific Peptidase 3 (USP3))

Alternative Name: USP3 / UBP

Background: Name/Gene ID: USP3
Subfamily: Cysteine C19
Family: Protease

Synonyms: USP3, Deubiquitinating enzyme 3, SIH003, Ubiquitin specific protease 3, UBP, Ubiquitin thiolesterase 3, Ubiquitin thioesterase 3, Ubiquitin specific peptidase 3

Gene ID: 9960

NCBI Accession: NP_006528

UniProt: Q9Y6I4