GREB1 antibody (AA 144-193)

Catalog No. ABIN6740395

The Rabbit Polyclonal anti-GREB1 antibody has been validated for WB. It is suitable to detect GREB1 in samples from Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Zebrafish (Danio rerio), Pig, Chicken, Monkey, Xenopus laevis and Bat.

Quick Overview for GREB1 antibody (AA 144-193) (ABIN6740395)

Target: GREB1 (Growth Regulation By Estrogen in Breast Cancer 1 (GREB1))

Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Zebrafish (Danio rerio), Pig, Chicken, Monkey, Xenopus laevis, Bat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GREB1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-GREB1 Antibody

Binding Specificity: AA 144-193

Specificity: Human GREB1

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Rabbit, Horse, Pig, Guinea pig, Chicken (100%) Zebrafish (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa144-193 of human GREB1 (Q4ZG55, NP_683701). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Platypus, Lizard, Xenopus (100%), Stickleback (92%), Zebrafish (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:312500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for GREB1

Target: GREB1 (Growth Regulation By Estrogen in Breast Cancer 1 (GREB1))

Alternative Name: GREB1

Background: Name/Gene ID: GREB1

Synonyms: GREB1, KIAA0575, Protein GREB1

Gene ID: 9687

NCBI Accession: NP_683701

UniProt: Q4ZG55