DDX1 antibody (AA 36-85)

Catalog No. ABIN6740488

The Rabbit Polyclonal anti-DDX1 antibody has been validated for WB. It is suitable to detect DDX1 in samples from Human, Mouse, Dog, Rabbit, Horse, Monkey, Pig, Bat and Xenopus laevis.

Quick Overview for DDX1 antibody (AA 36-85) (ABIN6740488)

Target: DDX1 (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 1 (DDX1))

Reactivity: Human, Mouse, Dog, Rabbit, Horse, Monkey, Pig, Bat, Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DDX1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-DDX1 Antibody

Binding Specificity: AA 36-85

Specificity: Human DDX1

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Dog, Rabbit, Horse, Xenopus (100%) Rat, Bovine (92%) Chicken (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa36-85 of human DDX1 (Q92499, NP_004930). Percent identity by BLAST analysis: Human, Chimpanzee, Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Dog, Bat, Rabbit, Horse, Pig, Opossum, Lizard, Xenopus (100%), Rat, Elephant, Bovine (92%), Turkey, Chicken (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:62500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for DDX1

Target: DDX1 (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 1 (DDX1))

Alternative Name: DDX1

Background: Name/Gene ID: DDX1
Family: DEAD box protein

Synonyms: DDX1, DEAD box polypeptide 1, DEAD box-1, DBP-RB, DEAD box protein 1, UKVH5d

Gene ID: 1653

NCBI Accession: NP_004930

UniProt: Q92499

Pathways: Ribonucleoprotein Complex Subunit Organization