BUD31 antibody (N-Term)

Catalog No. ABIN6740544

This Rabbit Polyclonal antibody specifically detects BUD31 in WB. It exhibits reactivity toward Human, Rat, Mouse, Cow, Zebrafish (Danio rerio), Dog, Horse, Rabbit, Guinea Pig, Bat, Chicken, Hamster, Monkey, Pig, Xenopus laevis and Drosophila melanogaster.

Quick Overview for BUD31 antibody (N-Term) (ABIN6740544)

Target: BUD31 (BUD31 Homolog (BUD31))

Reactivity: Human, Rat, Mouse, Cow, Zebrafish (Danio rerio), Dog, Horse, Rabbit, Guinea Pig, Bat, Chicken, Hamster, Monkey, Pig, Xenopus laevis, Drosophila melanogaster

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BUD31 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-BUD31 Antibody

Binding Specificity: N-Term

Specificity: Human BUD31

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Chicken, Zebrafish (100%) Xenopus (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from N-Terminus of human BUD31 (P41223, NP_003901). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Platypus, Xenopus, Frog, Smelt, Sablefish, Stickleback, Zebrafish, Drosophila, Nematode (100%), Beetle (92%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:312500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for BUD31

Target: BUD31 (BUD31 Homolog (BUD31))

Alternative Name: BUD31

Background: Name/Gene ID: BUD31

Synonyms: BUD31, BUD31 homolog (S. cerevisiae), BUD31 homolog (yeast), EDG-2, EDG2, FSAP17, G10, G10 protein homolog, Protein G10 homolog, Protein BUD31 homolog, Protein EDG-2, Maternal G10 transcript, YCR063W, Cwc14

Gene ID: 8896

NCBI Accession: NP_003901

UniProt: P41223