AGFG1 antibody (AA 179-228)

Catalog No. ABIN6740676

The Rabbit Polyclonal anti-AGFG1 antibody has been validated for WB. It is suitable to detect AGFG1 in samples from Human, Mouse, Rat, Guinea Pig, Horse, Chicken, Monkey and Pig.

Quick Overview for AGFG1 antibody (AA 179-228) (ABIN6740676)

Target: AGFG1 (HRB) (HIV-1 Rev Binding Protein (HRB))

Reactivity: Human, Mouse, Rat, Guinea Pig, Horse, Chicken, Monkey, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AGFG1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-AGFG1 Antibody

Binding Specificity: AA 179-228

Specificity: Human AGFG1

Predicted Reactivity: Percent identity by BLAST analysis: Human, Monkey, Mouse, Rat (100%) Bovine (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa179-228 of human AGFG1 (P52594, NP_004495). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Horse, Pig, Guinea pig, Chicken (100%), Opossum, Turkey, Zebra finch (93%), Dog, Bovine, Bat, Lizard (92%), Platypus (86%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:62500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for AGFG1

Target: AGFG1 (HRB) (HIV-1 Rev Binding Protein (HRB))

Alternative Name: AGFG1

Background: Name/Gene ID: AGFG1

Synonyms: AGFG1, HRIP, Rev interacting protein, HIV-1 Rev binding protein, HIV-1 Rev-binding protein, HRB, Rev-interacting protein, RIP, Nucleoporin-like protein RIP, RAB, ArfGAP with FG repeats 1

Gene ID: 3267

NCBI Accession: NP_004495

UniProt: P52594