UBE2D3 antibody (N-Term)

Catalog No. ABIN6740793

This Rabbit Polyclonal antibody specifically detects UBE2D3 in WB. It exhibits reactivity toward Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Chicken, Hamster and Monkey.

Quick Overview for UBE2D3 antibody (N-Term) (ABIN6740793)

Target: UBE2D3 (Ubiquitin-Conjugating Enzyme E2D 3 (UBE2D3))

Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Chicken, Hamster, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UBE2D3 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-UBE2D3 Antibody

Binding Specificity: N-Term

Specificity: Human UBE2D3

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Chicken (100%) Zebrafish (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from N-Terminus of human UBE2D3 (P61077, NP_003331). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Monkey, Galago, Marmoset, Mouse, Rat, Hamster, Elephant, Dog, Bovine, Horse, Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Platypus (100%), Gibbon, Bat, Pig, Lizard, Xenopus, Trout, Salmon, Smelt, Stickleback, Pike, Zebrafish (92%), Drosophila, Beetle (85%), Goat, Panda, Rabbit, Catfish, Nematode (84%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:1562500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for UBE2D3

Target: UBE2D3 (Ubiquitin-Conjugating Enzyme E2D 3 (UBE2D3))

Alternative Name: UBE2D3

Background: Name/Gene ID: UBE2D3

Synonyms: UBE2D3, E2(17)KB3, E2(17)KB 3, Ubiquitin-protein ligase D3, UBCH5C, UBC4/5, UBC5C, Ubiquitin carrier protein D3

Gene ID: 7323

NCBI Accession: NP_003331

UniProt: P61077

Pathways: Activation of Innate immune Response, Toll-Like Receptors Cascades