MARCH1 antibody (AA 36-85)

Catalog No. ABIN6740805

The Rabbit Polyclonal anti-MARCH1 antibody has been validated for WB. It is suitable to detect MARCH1 in samples from Human, Mouse, Rat, Cow, Guinea Pig, Horse, Rabbit, Bat, Chicken and Monkey.

Quick Overview for MARCH1 antibody (AA 36-85) (ABIN6740805)

Target: MARCH1 (Membrane-Associated Ring Finger (C3HC4) 1, E3 Ubiquitin Protein Ligase (MARCH1))

Reactivity: Human, Mouse, Rat, Cow, Guinea Pig, Horse, Rabbit, Bat, Chicken, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MARCH1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-MARCH1 Antibody

Binding Specificity: AA 36-85

Specificity: Human MARCH1

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat (100%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa36-85 of human MARCH1 (Q8TCQ1-2, NP_060393). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Elephant, Panda, Bovine, Bat, Rabbit, Horse, Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Platypus, Lizard (100%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:1562500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for MARCH1

Target: MARCH1 (Membrane-Associated Ring Finger (C3HC4) 1, E3 Ubiquitin Protein Ligase (MARCH1))

Alternative Name: MARCH1

Background: Name/Gene ID: MARCH1

Synonyms: MARCH1, MARCH-I, RING finger protein 171, RNF171

Gene ID: 55016

NCBI Accession: NP_060393

UniProt: Q8TCQ1