RNF182 antibody (AA 144-193)

Catalog No. ABIN6740825

The Rabbit Polyclonal anti-RNF182 antibody has been validated for WB. It is suitable to detect RNF182 in samples from Human, Mouse, Rat, Cow, Horse, Rabbit, Dog, Hamster and Monkey.

Quick Overview for RNF182 antibody (AA 144-193) (ABIN6740825)

Target: RNF182 (Ring Finger Protein 182 (RNF182))

Reactivity: Human, Mouse, Rat, Cow, Horse, Rabbit, Dog, Hamster, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RNF182 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-RNF182 Antibody

Binding Specificity: AA 144-193

Specificity: Human RNF182

Predicted Reactivity: Percent identity by BLAST analysis: Dog (100%) Bovine (86%) Chicken (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa144-193 of human RNF182 (Q8N6D2, NP_689950). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Hamster, Panda, Dog, Rabbit, Horse (100%), Bat, Pig, Guinea pig (92%), Bovine (86%), Opossum, Turkey, Zebra finch, Chicken, Platypus (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:62500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for RNF182

Target: RNF182 (Ring Finger Protein 182 (RNF182))

Alternative Name: RNF182

Background: Name/Gene ID: RNF182

Synonyms: RNF182, Ring finger protein 182

Gene ID: 221687

NCBI Accession: NP_689950

UniProt: Q8N6D2