HSPA4 antibody (AA 36-85)

Catalog No. ABIN6740952

The Rabbit Polyclonal anti-HSPA4 antibody has been validated for WB. It is suitable to detect HSPA4 in samples from Human, Mouse, Cow, Monkey, Dog, Horse, Guinea Pig, Rabbit, Pig, Chicken, Xenopus laevis and Bat.

Quick Overview for HSPA4 antibody (AA 36-85) (ABIN6740952)

Target: HSPA4 (Heat Shock 70kDa Protein 4 (HSPA4))

Reactivity: Human, Mouse, Cow, Monkey, Dog, Horse, Guinea Pig, Rabbit, Pig, Chicken, Xenopus laevis, Bat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HSPA4 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-HSPA4 Antibody

Binding Specificity: AA 36-85

Specificity: Human HSPA4

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Bovine, Rabbit, Horse, Pig, Guinea pig, Chicken, Xenopus (100%) Rat, Zebrafish (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa36-85 of human HSPA4 (P34932, NP_002145). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Turkey, Chicken, Platypus, Lizard, Xenopus (100%), Rat, Zebrafish (92%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:62500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for HSPA4

Target: HSPA4 (Heat Shock 70kDa Protein 4 (HSPA4))

Alternative Name: HSPA4 / APG-2

Background: Name/Gene ID: HSPA4

Synonyms: HSPA4, APG-2, APG2, Heat shock 70kD protein 4, Hsp70 RY, Hsp70RY, HSPH2, Hsp70, HS24/P52, RY, Heat shock 70 kDa protein 4, Heat shock 70  kDa protein 4, Heat shock protein, 110 kDa

Gene ID: 3308

NCBI Accession: NP_002145

UniProt: P34932