PDHB antibody (AA 72-121)

Catalog No. ABIN6741021

This anti-PDHB antibody is a Rabbit Polyclonal antibody detecting PDHB in WB. Suitable for Human, Rat, Mouse, Cow, Dog, Guinea Pig, Horse, Rabbit, Bat, Chicken, Monkey, Pig and Saccharomyces cerevisiae.

Quick Overview for PDHB antibody (AA 72-121) (ABIN6741021)

Target: PDHB (Pyruvate Dehydrogenase beta (PDHB))

Reactivity: Human, Rat, Mouse, Cow, Dog, Guinea Pig, Horse, Rabbit, Bat, Chicken, Monkey, Pig, Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PDHB antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-PDHB Antibody

Binding Specificity: AA 72-121

Specificity: Human PDHB

Predicted Reactivity: Percent identity by BLAST analysis: Human, Dog, Rabbit, Horse, Pig, Chicken (100%) Zebrafish (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa72-121 of human PDHB (P11177, NP_000916). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Mouse, Rat, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Turkey, Chicken, Lizard, Catfish (100%), Zebra finch, Salmon, Smelt, Stickleback, Pike, Pufferfish, Zebrafish, Drosophila (92%), Xenopus, Beetle (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:312500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for PDHB

Target: PDHB (Pyruvate Dehydrogenase beta (PDHB))

Alternative Name: PDHB

Background: Name/Gene ID: PDHB

Synonyms: PDHB, PDHE1-B, PDHBD, PHE1B

Gene ID: 5162

NCBI Accession: NP_000916

UniProt: P11177

Pathways: Warburg Effect