ATP5F1 antibody (AA 180-229)

Catalog No. ABIN6741025

This anti-ATP5F1 antibody is a Rabbit Polyclonal antibody detecting ATP5F1 in WB. Suitable for Human, Mouse, Cow, Dog, Guinea Pig, Rabbit and Monkey.

Quick Overview for ATP5F1 antibody (AA 180-229) (ABIN6741025)

Target: ATP5F1 (ATP Synthase, H+ Transporting, Mitochondrial Fo Complex, Subunit B1 (ATP5F1))

Reactivity: Human, Mouse, Cow, Dog, Guinea Pig, Rabbit, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATP5F1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-ATP5F1 Antibody

Binding Specificity: AA 180-229

Specificity: Human ATP5F1

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Dog (100%) Pig (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa180-229 of human ATP5F1 (P24539, NP_001679). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Marmoset, Mouse, Elephant, Panda, Dog, Bovine, Rabbit, Guinea pig (100%), Orangutan, Rat, Horse, Pig (92%), Monkey, Opossum, Platypus (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:12500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for ATP5F1

Target: ATP5F1 (ATP Synthase, H+ Transporting, Mitochondrial Fo Complex, Subunit B1 (ATP5F1))

Alternative Name: ATP5F1

Background: Name/Gene ID: ATP5F1
Subfamily: ATPase - F type
Family: Transporter

Synonyms: ATP5F1, ATPase subunit b, H+-ATP synthase subunit b, PIG47

Gene ID: 515

NCBI Accession: NP_001679

UniProt: P24539

Pathways: Proton Transport, Ribonucleoside Biosynthetic Process