IDH2 antibody (AA 144-193)

Catalog No. ABIN6741027

This anti-IDH2 antibody is a Rabbit Polyclonal antibody detecting IDH2 in WB. Suitable for Human, Mouse, Rat, Pig, Monkey, Cow, Dog, Horse, Rabbit, Guinea Pig, Zebrafish (Danio rerio), Bat, Hamster, Chicken and Xenopus laevis.

Quick Overview for IDH2 antibody (AA 144-193) (ABIN6741027)

Target: IDH2 (Isocitrate Dehydrogenase 2 (NADP+), Mitochondrial (IDH2))

Reactivity: Human, Mouse, Rat, Pig, Monkey, Cow, Dog, Horse, Rabbit, Guinea Pig, Zebrafish (Danio rerio), Bat, Hamster, Chicken, Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IDH2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-IDH2 Antibody

Binding Specificity: AA 144-193

Specificity: Human IDH2

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Dog, Rabbit, Pig, Chicken, Xenopus (100%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa144-193 of human IDH2 (P48735, NP_002159). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Hamster, Elephant, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Chicken, Platypus, Xenopus, Stickleback, Zebrafish, Sea squirt, Slime mold (100%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:12500.

Not recommended for: IHC-P

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for IDH2

Target: IDH2 (Isocitrate Dehydrogenase 2 (NADP+), Mitochondrial (IDH2))

Alternative Name: IDH / IDH2

Background: Name/Gene ID: IDH2

Synonyms: IDH2, D2HGA2, ICD-M, IDP, IDPM, MNADP-IDH, NADP(+)-specific ICDH, IDH, IDHM, Isocitrate dehydrogenase, Oxalosuccinate decarboxylase

Gene ID: 3418

NCBI Accession: NP_002159

UniProt: P48735

Pathways: Warburg Effect