IDH2 antibody (Middle Region)

Catalog No. ABIN2783282

There is 1 publication for this product available. The Rabbit Polyclonal anti-IDH2 antibody is suitable to detect IDH2 in samples from Human, Mouse, Rat, Cow, Dog, Horse, Rabbit, Guinea Pig, Zebrafish (Danio rerio) and Saccharomyces cerevisiae. It has been validated for WB.

Quick Overview for IDH2 antibody (Middle Region) (ABIN2783282)

Target: IDH2 (Isocitrate Dehydrogenase 2 (NADP+), Mitochondrial (IDH2))

Reactivity: Human, Mouse, Rat, Cow, Dog, Horse, Rabbit, Guinea Pig, Zebrafish (Danio rerio), Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IDH2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-IDH2 Antibody

Binding Specificity: Middle Region

Purpose: IDH2 Antibody - middle region

Sequence: GGTVFREPII CKNIPRLVPG WTKPITIGRH AHGDQYKATD FVADRAGTFK

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Yeast: 93%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against IDH2. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human IDH2

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP48207-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-IDH2 antibody (ABIN2783282)

Vohwinkel, Lecuona, Sun, Sommer, Vadász, Chandel, Sznajder: "Elevated CO(2) levels cause mitochondrial dysfunction and impair cell proliferation." in: The Journal of biological chemistry, Vol. 286, Issue 43, pp. 37067-76, (2011) (PubMed).

Target Details for IDH2

Target: IDH2 (Isocitrate Dehydrogenase 2 (NADP+), Mitochondrial (IDH2))

Alternative Name: IDH2

Background:

Background Information: Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. IDH2 is the NADP(+)-dependent isocitrate dehydrogenase found in the mitochondria. It plays a role in intermediary metabolism and energy production. This protein may tightly associate or interact with the pyruvate dehydrogenase complex.Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the mitochondria. It plays a role in intermediary metabolism and energy production. This protein may tightly associate or interact with the pyruvate dehydrogenase complex. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Gene Name: Isocitrate dehydrogenase 2 (NADP+), mitochondrial

Alternative Symbols: IDH, IDP, IDHM, IDPM, ICD-M, D2HGA2, mNADP-IDH

Protein Name: Isocitrate dehydrogenase [NADP], mitochondrial

Molecular Weight: 47kDa

Gene ID: 3418

NCBI Accession: NP_002159

UniProt: P48735

Pathways: Warburg Effect