C1orf177 antibody (AA 251-300)
This Rabbit Polyclonal antibody specifically detects C1orf177 in WB. It exhibits reactivity toward Human, Cow, Dog and Horse.
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Catalog No. ABIN6741217
Quick Overview for C1orf177 antibody (AA 251-300) (ABIN6741217)
Target
C1orf177 (C1ORF177)
(Chromosome 1 Open Reading Frame 177 (C1ORF177))
Reactivity
Human, Cow, Dog, Horse
Host
Rabbit
Clonality
Polyclonal
Conjugate
This C1orf177 antibody is un-conjugated
Application
Western Blotting (WB)
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Binding Specificity
- AA 251-300
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Specificity
- Human C1orf177
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Predicted Reactivity
- Percent identity by BLAST analysis: Rabbit (92%) Bovine, Horse (85%).
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Purification
- Immunoaffinity purified
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Immunogen
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Synthetic peptide located between aa251-300 of human C1orf177 (Q3ZCV2, NP_689820). Percent identity by BLAST analysis: Human, Gibbon, Monkey (100%), Gorilla, Galago, Marmoset, Rabbit (92%), Elephant, Bovine, Horse (85%), Mouse, Rat (78%).
Type of Immunogen: Synthetic peptide -
Isotype
- IgG
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Application Notes
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Approved: WB (0.2 - 1 μg/mL)
Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:1562500. -
Comment
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Target Species of Antibody: Human
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Distilled water
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Concentration
- Lot specific
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Buffer
- Lyophilized from PBS with 2 % sucrose
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Handling Advice
- Avoid repeat freeze-thaw cycles.
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Storage
- 4 °C,-20 °C
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Storage Comment
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Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
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