TMEM74 antibody (AA 215-264)

Catalog No. ABIN6741230

The Rabbit Polyclonal anti-TMEM74 antibody has been validated for WB. It is suitable to detect TMEM74 in samples from Human, Cow, Dog, Horse, Rabbit, Bat, Hamster and Monkey.

Quick Overview for TMEM74 antibody (AA 215-264) (ABIN6741230)

Target: TMEM74 (Transmembrane Protein 74 (TMEM74))

Reactivity: Human, Cow, Dog, Horse, Rabbit, Bat, Hamster, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TMEM74 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-TMEM74 Antibody

Binding Specificity: AA 215-264

Specificity: Human TMEM74

Predicted Reactivity: Percent identity by BLAST analysis: Bovine (100%) Rat (92%) Chicken (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa215-264 of human TMEM74 (Q96NL1, NP_694560). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset, Hamster, Elephant, Dog, Bovine, Bat, Rabbit, Horse (100%), Galago, Mouse, Rat, Panda, Pig, Guinea pig (92%), Opossum, Turkey, Zebra finch, Chicken, Lizard, Salmon, Stickleback, Zebrafish (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:62500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for TMEM74

Target: TMEM74 (Transmembrane Protein 74 (TMEM74))

Alternative Name: TMEM74

Background: Name/Gene ID: TMEM74

Synonyms: TMEM74, Transmembrane protein 74, NET36

Gene ID: 157753

NCBI Accession: NP_694560

UniProt: Q96NL1