DCAF4 antibody (AA 216-265)

Catalog No. ABIN6741305

The Rabbit Polyclonal anti-DCAF4 antibody has been validated for WB. It is suitable to detect DCAF4 in samples from Human, Pig and Monkey.

Quick Overview for DCAF4 antibody (AA 216-265) (ABIN6741305)

Target: DCAF4 (DDB1 and CUL4 Associated Factor 4 (DCAF4))

Reactivity: Human, Pig, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DCAF4 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-DCAF4 Antibody

Binding Specificity: AA 216-265

Specificity: Human DCAF4 / WDR21A

Predicted Reactivity: Percent identity by BLAST analysis: Dog (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa216-265 of human DCAF4 (Q8IV10, NP_851937). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset, Pig (100%), Panda, Dog, Horse, Opossum (92%), Rat, Bovine, Guinea pig, Zebra finch, Chicken (85%), Mouse (78%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:312500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for DCAF4

Target: DCAF4 (DDB1 and CUL4 Associated Factor 4 (DCAF4))

Alternative Name: WDR21A / DCAF4

Background: Name/Gene ID: DCAF4

Synonyms: DCAF4, WD repeat domain 21, WDR21, WDR21A, WD repeat domain 21A

Gene ID: 26094

NCBI Accession: NP_851937

UniProt: Q8WV16