Sphingomyelin Synthase 1 antibody (AA 144-193)

Catalog No. ABIN6741336

The Rabbit Polyclonal anti-Sphingomyelin Synthase 1 antibody has been validated for WB. It is suitable to detect Sphingomyelin Synthase 1 in samples from Human, Mouse, Rat, Rabbit, Cow, Dog, Guinea Pig, Horse, Bat, Monkey and Pig.

Quick Overview for Sphingomyelin Synthase 1 antibody (AA 144-193) (ABIN6741336)

Target: Sphingomyelin Synthase 1 (SGMS1)

Reactivity: Human, Mouse, Rat, Rabbit, Cow, Dog, Guinea Pig, Horse, Bat, Monkey, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Sphingomyelin Synthase 1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Sphingomyelin Synthase 1 Antibody

Binding Specificity: AA 144-193

Specificity: Human SGMS1

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Horse, Pig, Guinea pig (100%) Chicken (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa144-193 of human SGMS1 (Q86VZ5, NP_671512). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Dog, Bovine, Bat, Rabbit, Horse, Pig, Guinea pig, Platypus (100%), Turkey, Zebra finch, Chicken (92%), Opossum, Xenopus (84%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:62500.

Not recommended for: IHC-P

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for Sphingomyelin Synthase 1

Target: Sphingomyelin Synthase 1 (SGMS1)

Alternative Name: SGMS1 / TMEM23

Background: Name/Gene ID: SGMS1

Synonyms: SGMS1, Hmob33, MOB, MOB1, Sphingomyelin synthase 1, TMEM23, Transmembrane protein 23, Protein Mob, SMS1

Gene ID: 259230

NCBI Accession: NP_671512

UniProt: Q86VZ5

Pathways: Cellular Response to Molecule of Bacterial Origin