Sphingomyelin Synthase 1 antibody (Middle Region)

Catalog No. ABIN2786225

There is 1 publication for this product available. The Rabbit Polyclonal anti-Sphingomyelin Synthase 1 antibody is suitable to detect Sphingomyelin Synthase 1 in samples from Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit and Zebrafish (Danio rerio). It has been validated for WB.

Quick Overview for Sphingomyelin Synthase 1 antibody (Middle Region) (ABIN2786225)

Target: Sphingomyelin Synthase 1 (SGMS1)

Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Sphingomyelin Synthase 1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Sphingomyelin Synthase 1 Antibody

Binding Specificity: Middle Region

Purpose: Sgms1 Antibody - middle region

Sequence: LTTVMISVVH ERVPPKEVQP PLPDTFFDHF NRVQWAFSIC EINGMILVGL

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 77%

Characteristics: This is a rabbit polyclonal antibody against Sgms1. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of Mouse Sgms1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP55476-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-Sphingomyelin Synthase 1 antibody (ABIN2786225)

Miyamoto, Hsu, Hamm, Darshi, Diamond-Stanic, Declèves, Slater, Pennathur, Stauber, Dorrestein, Sharma: "Mass Spectrometry Imaging Reveals Elevated Glomerular ATP/AMP in Diabetes/obesity and Identifies Sphingomyelin as a Possible Mediator." in: EBioMedicine, Vol. 7, pp. 121-34, (2016) (PubMed).

Target Details for Sphingomyelin Synthase 1

Target: Sphingomyelin Synthase 1 (SGMS1)

Alternative Name: Sgms1

Background:

Background Information: Bidirectional lipid cholinephosphotransferase is capable of converting phosphatidylcholine (PC) and ceramide to sphingomyelin (SM) and diacylglycerol (DAG) and vice versa. Direction is dependent on the relative concentrations of DAG and ceramide as phosphocholine acceptors. Sgms1 directly and specifically recognizes the choline head group on the substrate. It also requires two fatty chains on the choline-P donor molecule in order to be recognized efficiently as a substrate. Sgms1 does not function strictly as a SM synthase and suppresses BAX-mediated apoptosis and also prevents cell death in response to stimuli such as hydrogen peroxide, osmotic stress, elevated temperature and exogenously supplied sphingolipids. Sgms1 may protect against cell death by reversing the stress-inducible increase in levels of proapoptotic ceramide.

Alternative Symbols: SM, Mob, Sms1, Sor1, SMS1b, SMS1g, C80702, Tmem23, AI841905, 9530058O11Rik

Protein Name: Phosphatidylcholine:ceramide cholinephosphotransferase 1

Molecular Weight: 49 kDa

Gene ID: 208449

NCBI Accession: NP_659041

UniProt: Q8VCQ6

Pathways: Cellular Response to Molecule of Bacterial Origin