SPAG8 antibody (AA 396-445)

Catalog No. ABIN6741363

The Rabbit Polyclonal anti-SPAG8 antibody has been validated for WB. It is suitable to detect SPAG8 in samples from Human, Mouse, Rat and Monkey.

Quick Overview for SPAG8 antibody (AA 396-445) (ABIN6741363)

Target: SPAG8 (Sperm Associated Antigen 8 (SPAG8))

Reactivity: Human, Mouse, Rat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SPAG8 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-SPAG8 Antibody

Binding Specificity: AA 396-445

Specificity: Human SPAG8

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat (100%) Rabbit (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa396-445 of human SPAG8 (Q99932-2, NP_758516). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Mouse, Rat (100%), Rabbit (92%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:62500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for SPAG8

Target: SPAG8 (Sperm Associated Antigen 8 (SPAG8))

Alternative Name: HSD-1 / SPAG8

Background: Name/Gene ID: SPAG8

Synonyms: SPAG8, Sperm membrane protein BS-84, Sperm-associated antigen 8, SMP-1, Sperm associated antigen 8, SMP1, SPAG3, BS-84, HSD-1, HSMP-1, Sperm membrane protein 1

Gene ID: 26206

NCBI Accession: NP_758516

UniProt: Q99932