SPATA7 antibody (AA 252-301)

Catalog No. ABIN6741401

The Rabbit Polyclonal anti-SPATA7 antibody has been validated for WB. It is suitable to detect SPATA7 in samples from Human, Rat, Dog, Horse, Pig, Rabbit, Bat and Monkey.

Quick Overview for SPATA7 antibody (AA 252-301) (ABIN6741401)

Target: SPATA7 (Spermatogenesis Associated 7 (SPATA7))

Reactivity: Human, Rat, Dog, Horse, Pig, Rabbit, Bat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SPATA7 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-SPATA7 Antibody

Binding Specificity: AA 252-301

Specificity: Human SPATA7

Predicted Reactivity: Percent identity by BLAST analysis: Human, Horse, Pig (100%) Mouse, Bovine, Guinea pig (92%) Rat, Dog (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa252-301 of human SPATA7 (Q9P0W8, NP_060888). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Monkey, Galago, Marmoset, Bat, Horse, Pig, Opossum (100%), Mouse, Bovine, Guinea pig (92%), Elephant (90%), Rat, Dog, Platypus (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:1562500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for SPATA7

Target: SPATA7 (Spermatogenesis Associated 7 (SPATA7))

Alternative Name: LCA3 / SPATA7

Background: Name/Gene ID: SPATA7

Synonyms: SPATA7, HSD-3.1, Leber congenital amaurosis 3, HSD3, LCA3, Spermatogenesis associated 7

Gene ID: 55812

NCBI Accession: NP_060888

UniProt: Q9P0W8