RNF5 antibody (N-Term)

Catalog No. ABIN6741432

This anti-RNF5 antibody is a Rabbit Polyclonal antibody detecting RNF5 in WB. Suitable for Human, Mouse, Rat, Cow, Dog, Pig and Monkey.

Quick Overview for RNF5 antibody (N-Term) (ABIN6741432)

Target: RNF5 (Ring Finger Protein 5, E3 Ubiquitin Protein Ligase (RNF5))

Reactivity: Human, Mouse, Rat, Cow, Dog, Pig, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RNF5 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-RNF5 Antibody

Binding Specificity: N-Term

Specificity: Human RNF5

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Pig (100%) Guinea pig (92%) Rabbit (91%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from N-Terminus of human RNF5 (Q99942, NP_008844). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bovine, Pig (100%), Hamster, Horse, Guinea pig (92%), Rabbit (91%), Bat (85%), Opossum (84%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:12500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for RNF5

Target: RNF5 (Ring Finger Protein 5, E3 Ubiquitin Protein Ligase (RNF5))

Alternative Name: RMA1 / RNF5

Background: Name/Gene ID: RNF5

Synonyms: RNF5, G16, HsRma1, NG2, RING5, Protein G16, Ram1 homolog, RING finger protein 5, RMA1

Gene ID: 6048

NCBI Accession: NP_008844

UniProt: Q99942